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cultrex reduced growth factor basement membrane extract  (R&D Systems)


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    R&D Systems cultrex reduced growth factor basement membrane extract
    Cultrex Reduced Growth Factor Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cultrex+reduced+growth+factor+basement+membrane+extract/Cultrex+Reduced+Growth+Factor+Basement+Membrane+Extract%2C+Type+2%2C+Select/pmc13112633-64-6-15
    Average 96 stars, based on 244 article reviews
    cultrex reduced growth factor basement membrane extract - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Brief report on the development of patient-derived lung cancer organoids with keratinizing squamous cell carcinoma morphology
    Article Snippet: 1 h at 37°C in DMEM/F-12 (Thermo Fisher Scientific, Waltham, Massachusetts) supplemented with 1% Antibiotic-Antimycotic, 1 mg mL -1 collagenase (Sigma-Aldrich, Burlington, Massachusetts), and 0.001% DNAse (Sigma-Aldrich). .. The dissociated tumour solution was passed through a 100 μm strainer and tumour cells were pelleted, counted, and seeded at 80,000 cells per 50 μL dome in DMEM/F-12 + Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R & D Systems, Minneapolis, Minnesota) (1:10). ..

    Article Title: A modular silk-collagen scaffold to model dorsoventral spinal cord organization using human pluripotent stem cells.
    Article Snippet: In vitro tissue models are critical to our understanding of human cell functions and interactions, but their limited complexity can hinder translation to in vivo systems.. Bioengineered 3D tissues are gradually improving the capabilities of in vitro models, but the highly complex spatial organization of the human central nervous system (CNS) represents a particular challenge.. Many 3D CNS models are limited to single cell types, while multicellular models generally lack control of cell organization, failing to recapitulate the regional specificity of cells in vivo.

    Article Title: Low-Cost, Rapid Fabrication of Customizable Polyethylene Glycol-Based Cell Culture Devices.
    Article Snippet: Biological research groups may face a high barrier to entry when constructing custom 3D cell culture devices to investigate multi-tissue interactions in vitro.. Standard fabrication methods such as lithography, etching, or molding are expensive and require specialized equipment and expertise.. To address this, we developed an accessible approach for producing polyethylene glycol (PEG)-based cell culture devices using stereolithography 3D printing with a polydimethylsiloxane intermediate mold.

    Article Title: Evaluation of the efficiency of double recombinant vaccinia virus VV-GMCSF-Lact against glioblastoma using 3D spheroid and cerebral organoid models
    Article Snippet: On day 7, the medium was switched to neural induction medium consisting of DMEM/F12 with N-2 Supplement (Thermo Fisher Scientific, USA), 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin–streptomycin, and 1 μg/mL heparin (Sigma-Aldrich, USA). .. On day 11, COs were embedded in 10 μL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D System, USA) and cultured in CO differentiation medium without Vitamin A (DMEM/F12–neurobasal medium 1:1, 0.5× N-2 supplement, 1× B-27 supplement minus Vitamin A [Thermo Fisher Scientific, USA], 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin– streptomycin, and 2.5 μg/mL insulin (Sigma-Aldrich, USA]) in anti-adherence-treated dishes. .. On day 15, COs were removed from the Cultrex extract and transferred to differentiation medium with Vitamin A (DMEM/ F12–neurobasal medium 1:1, 0.5× N-2 supplement, 1× B-27 supplement [Thermo Fisher Scientific, USA], 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin–streptomycin, 2.5 μg/mL insulin, 0.8 mM ascorbic acid (FUJIFILM Wako Pure Chemical Corporation, JAPAN], and 20 mM N-2-hydroxyethylpiperazine-N2-ethanesulfonic acid [HEPES; Capricorn Scientific, Germany]), and cultured on a CO2 incubator shaker at 80 rpm with medium changes every 4 days.

    Article Title: Enhancing cancer killing and natural killer cell persistence by targeting NOXA, a predictor of poor patient survival
    Article Snippet: To enable organoid-specific detection in co-culture experiments, SNU-3947-TO were transduced with a lentiviral vector encoding tdTomato and luciferase (pDRM18-LTN, Addgene #174721). .. Transduced organoid was expanded in Cultrex Reduced Growth Factor Basement Membrane Extract (BME; R&D Systems, Systems, Minneapolis, MN, USA). .. For experimental assays, organoids were resuspended in 100 μL BME and seeded at 20 μL per well in low-attachment CellStar 48-well plates (Greiner Bio-one, Monroe, NC, USA) under standard organoid growth conditions.

    Article Title: Eutopic echoes ectopic: organoid-based evidence of shared hormone dysregulation in endometriosis
    Article Snippet: Persistent erythrocytes were depleted with 5 mL Red Blood Cell Lysis Buffer (Roche). .. After pelleting, cells were resuspended in Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2 (R&D Systems) and seeded in prewarmed 24 (48)-well plates (Falcon) with ~ 60,000 (~ 30,000) cells/well in a 50 μl (25 μl) drop. ..

    Article Title: Application of Chorionic Villus Sampling to Longitudinal Studies in Pregnant Non-Human Primate Models.
    Article Snippet: .. Cells were suspended in Cultrex reduced growth factor basement membrane extract, and type R1 (R&D Systems, Minneapolis, MN, USA, 3433-005-R1) and 25 μL droplets were seeded into a 48-well plate. ..

    Article Title: Application of Chorionic Villus Sampling to Longitudinal Studies in Pregnant Non-Human Primate Models
    Article Snippet: .. Cells were suspended in Cultrex reduced growth factor basement membrane extract, and type R1 (R&D Systems, Minneapolis, MN, USA, 3433-005-R1) and 25 μL droplets were seeded into a 48-well plate. ..

    Passaging:

    Article Title: Low-Cost, Rapid Fabrication of Customizable Polyethylene Glycol-Based Cell Culture Devices.
    Article Snippet: Biological research groups may face a high barrier to entry when constructing custom 3D cell culture devices to investigate multi-tissue interactions in vitro.. Standard fabrication methods such as lithography, etching, or molding are expensive and require specialized equipment and expertise.. To address this, we developed an accessible approach for producing polyethylene glycol (PEG)-based cell culture devices using stereolithography 3D printing with a polydimethylsiloxane intermediate mold.

    Cell Culture:

    Article Title: Evaluation of the efficiency of double recombinant vaccinia virus VV-GMCSF-Lact against glioblastoma using 3D spheroid and cerebral organoid models
    Article Snippet: On day 7, the medium was switched to neural induction medium consisting of DMEM/F12 with N-2 Supplement (Thermo Fisher Scientific, USA), 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin–streptomycin, and 1 μg/mL heparin (Sigma-Aldrich, USA). .. On day 11, COs were embedded in 10 μL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D System, USA) and cultured in CO differentiation medium without Vitamin A (DMEM/F12–neurobasal medium 1:1, 0.5× N-2 supplement, 1× B-27 supplement minus Vitamin A [Thermo Fisher Scientific, USA], 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin– streptomycin, and 2.5 μg/mL insulin (Sigma-Aldrich, USA]) in anti-adherence-treated dishes. .. On day 15, COs were removed from the Cultrex extract and transferred to differentiation medium with Vitamin A (DMEM/ F12–neurobasal medium 1:1, 0.5× N-2 supplement, 1× B-27 supplement [Thermo Fisher Scientific, USA], 1 mM L-glutamine, 1% MEM NEAA, 0.1 mM 2-mercaptoethanol, 1% penicillin–streptomycin, 2.5 μg/mL insulin, 0.8 mM ascorbic acid (FUJIFILM Wako Pure Chemical Corporation, JAPAN], and 20 mM N-2-hydroxyethylpiperazine-N2-ethanesulfonic acid [HEPES; Capricorn Scientific, Germany]), and cultured on a CO2 incubator shaker at 80 rpm with medium changes every 4 days.



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